Affiliation:
1. Federal University of Sergipe (UFS)
2. Federal University of Minas Gerais (UFMG)
3. University of Coimbra
Abstract
Abstract
Background: Plants of the Myrcia genus have been widely used in folk medicine to treat various diseases. The Myrcia splendens species has a diverse chemical constitution, but the biological activities of its essential oil have not been well investigated.
Objective: To carry out the chemistry characterization and evaluate the in vitroantitumor effect of the essential oil from the leaves of the species Myrcia splendens (MSEO) in different tumor lines.
Methods: The essential oil obtained from M. splendens (MSEO) leaves was analyzed by GC/MS. The cytotoxic activity of the MSEO was evaluated in vitro using the MTT assay. Clonogenic and wound healing assays were used to analyze colony formation and migratory capacity of lung adenocarcinoma cells, respectively. Morphological changes were observed by fluorescence using Phalloidin/FITC and DAPI stains.
Results: Chemical analysis identified 22 compounds, corresponding to 88% of the sample. Major compounds were the sesquiterpenic hydrocarbons bicyclogermacrene (15.4%), germacrene D (8.9%) and E-caryophyllene (10.1%). MSEO was highly cytotoxic in A549, B16-F10 and THP-1 cancer cells. A549 human lung adenocarcinoma cell line was chosen to continue the experiments, due to the high incidence and mortality rates of this type of cancer. MSEO reduced colony formation of at all concentrations and inhibited the migratory capacity of A549 cells at concentrations of 20 and 40 ug/mL. Furthemore, the MSEO induced apoptotic in A549 cells at all concentrations tested.
Conclusion: MSEO may be a promising natural agent in anti-cancer applications.
Publisher
Research Square Platform LLC
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