The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse

Abstract

Abstract Allium jesdianum Boiss. & Buhse. is a pharmaceutically valuable plant that is threatened with extinction due to over-exploitation of wild plants. In vitro culture of plants is a favorable alternative for the conservation of endangered plant species. To establish an efficient protocol for micropropagation of Allium jesdianum, the effects of plant growth regulators, basal medium, and light were studied in this experiment. Basal parts of collected bulbs from Zagros Mountains in Iran were surface sterilized and placed on five different basal media with different concentrations of IBA, NAA, BAP and kinetin and kept under dark and light conditions. The results showed that among the exogenously applied PGRs, IBA at concentrations of 1 and 2 mg/l was the most convincing PGR for shoot and bulblet regeneration. The best results were obtained with CLC and MS media under light. Higher NH4+ also increased regeneration. Our experiment may have implications for plant growth and conservation, as well as for in vitrobreeding and drug development.