Non-classical CD45RBlo memory B-cells are the majority of circulating antigen-specific B-cells following mRNA vaccination and COVID-19 infection.

Author:

Priest David G.1,Ebihara Takeshi1,Tulyeu Janyerkye1ORCID,Søndergaard Jonas1ORCID,Sakakibara Shuhei2ORCID,Sugihara Fuminori1,Nakao Shunichiro1,Togami Yuki1,Yoshimura Jumpei1,Ito Hiroshi1,Onishi Shinya1,Muratsu Arisa1,Mitsuyama Yumi1,Ogura Hiroshi1,Oda Jun1ORCID,Okuzaki Daisuke3ORCID,Matsumoto Hisatake4,Wing James B.1ORCID

Affiliation:

1. Osaka University

2. Immunology Frontier Research Center, Osaka University

3. Single Cell Genomics, Human immunology, WPI Immunology Frontier Research Center, Osaka University

4. Osaka University Graduate School of Medicine

Abstract

Abstract

Resting memory B-cells can be divided into classical and non-classical groups based on differential expression of markers such as CD27 and CD11c, while activated memory B-cells express a combination of markers, making their ontogeny hard to determine. Here by longitudinal analysis of COVID-19, bacterial sepsis, and BNT162b2 mRNA vaccine recipients by mass cytometry and CITE-seq we describe a three-branch structure of resting B-cell memory consisting of “classical” CD45RB+ memory and two branches of CD45RBlo memory further defined by expression of CD23 and CD11c respectively. Stable differences in CD45RB upon activation allowed tracking of activated B-cells and plasmablasts derived from CD45RB+ classical and CD45RBlo non-classical memory B-cells. In both COVID-19 patients and mRNA vaccination, CD45RBlo B-cells formed the majority of SARS-CoV2 specific memory B-cells and correlated with serum antibodies while CD45RB+ memory was most strongly activated by bacterial Sepsis. These results suggest that diverse non-classical CD45RBlo memory B-cells consisting of branches of CD11c+Tbet+ and CD23+ fractions form a critical part of responses to viral infection and vaccination.

Publisher

Springer Science and Business Media LLC

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