Gene Polymorphism of KLF14 rs972283 and Its Association with Type 2 Diabetes Mellitus in the Egyptian patients

Abstract

Abstract Background: An extensive quest for genetic variables impacting the susceptibility for T2DM and other cardiometabolic disorders has been sparked by the global growth in the frequency of those widespread ailments. In Egypt, no previous studies examined the relation between KLF14 rs972283 SNP and the risk of developing T2DM. This study aims to ascertain the genetic influences of KLF14 rs972283 on T2DM in Egypt. Subjects and methods: This study included 50 diabetic patients and 50 healthy subjects. Each individual underwent a clinical assessment and total body examination, laboratory investigations including liver enzymes, FBS, HbA1C, and lipid profile. The DNA Purification Kit was used to separate genomic DNA from the whole blood. Gene polymorphism was detected via the PCR-REFLP method. Results: There was no association between KLF14 (rs972283) gene polymorphism and T2DM among studied people. The distribution of KLF14 rs972283 genotypes in diabetic cases was 9 patients with GG genotype (18%) and 26 with GA (52%) while 15 patients (30%) had homozygous mutant genotype AA. However, in the control group, 7 healthy cases had the GG genotype (14%), 31 (62%) had the GA genotypes, and 12 (24%) had the AA genotype. Concerning the allele frequency, the A allele in the KLF14 rs972283 polymorphism was more frequent in both diabetic and control cases (56% and 55%; respectively), but without significant difference (P=0.887). Conclusion: The present study shows for the first time that rs972283 SNP of the KLF14 gene was not associated with T2DM susceptibility in the Egyptian population. However, this study detects an association between KLF14 rs972283 (GG genotype) and a higher risk for elevated TG and LDL levels in control cases. This could indicate that the presence of the GG genotype even in control cases could be used to predict cardiovascular disease.