MiR-185-5p Inhibits the Proliferation, Migration and Invasion of Gastric Carcinoma Cells Through Targeting the SMAD3 Pathway

Abstract

Abstract Objective: To explore the effects of miR-185-5p on the migration, invasion and proliferation of human gastric carcinoma HGC-27 cells. Methods: HGC-27 cells cultured to the logarithmic growth phase were divided into NC mimic group, miR-185-5p mimic (miR-mimic) group, NC inhibitor group and miR-185-5p inhibitor (miR-inhibitor) group. The changes in the migration and invasion capabilities of HGC-27 cells were detected by wound healing assay and Transwell assays, respectively. The viability and proliferation capability of HGC-27 cells in each group were assessed by CCK-8 assay and colony formation assay, respectively. The relative protein expressions of TGF-β1, SMAD3, MMP2, MMP3, MMP9, Cyclin D1, PCNA and P21 in HGC-27 cells were determined using Western blotting. Results: After HGC-27 cells were transfected with miR-inhibitor, the protein expressions of MMP2, MMP3, MMP9, Cyclin D1, PCNA and P21 all rose,and the relative protein expression of TGF-β1 and SMAD3 decreased, and the migration distance of HGC-27 cells, the numbers of cells passing through the lower chamber and the basement membrane, the cell viability and the number of colonies formed were significantly increased. However, the above phenomena were reversed after overexpression of miR-185-5p in vitro. Conclusion: The overexpression of miR-185-5p can, through targeting the SMAD3 pathway, inhibit the proliferation, migration and invasion of GC cells.