Scavenger Receptor Class B Type 1 Gene Polymorphism as a Possible Genetic Risk Factor for Insulin resistance

Abstract

Abstract The association between SCARB1 rs74830677 (c.1127 C > T, [P.pro 367 leu] P367L) gene polymorphisms and insulin resistance (IR) has been not been analysed and studied. The clinical relevance of these findings remained uncertain and brings into question the importance of SCARB1 in human physiology. So, we investigated the hypothesis the SCARB1 SNPs in association with the risk of developing insulin resistance. A total of 600 individuals, including 300 T2DM and 300 healthy individuals, were enrolled in the study. Blood samples were collected from each T2DM and healthy individual. CT method for the relative quantitation were used for gene expression level in T2DM and healthy controls. Also, restriction fragment length polymorphism analysis was achieved to detect the incidence of genetic polymorphisms. HOMA resistance index and QUICKI were calculated from fasting plasma glucose and insulin values. In the present study, there was strong association observed between T2DM and SCARB1 rs74830677 gene polymorphism (OR = 1.7, 95% CI 1.18–2.71, P = 0.07). Further, T allele were associated with increase serum insulin and HOMA-IR. Our genotyping data showed that SCARB1 rs7480677 variant is significantly higher in subjects with increased HOMA-IR. Thus, we conclude that SCARB1 polymorphisms may play a crucial role in pathogenesis and susceptibility of insulin resistance thus leads to the development of type 2 diabetes mellitus.