Simulated nitrogen deposition altered the differences in leaf traits among seasons and plant types along the vertical gradient in an evergreen broadleaf forest of western China

Author:

Chang Wenzheng,Liu Sining1ORCID,Zhang Luyao,Song Qiu,Liang Tianxing,Chen Jie,Wang Zimeng,Xia Yingying,Qubi Niangniang,Zheng Xiaoying,Xiao Yuxi,Wang Lixia,Li Han,Zhang Li,You Chengming,Xu Hongwei,Xu Lin,Tan Bo,Xu Zhenfeng

Affiliation:

1. Sichuan Agricultural University College of Forestry

Abstract

Abstract

Background and Aims Previous studies have focused on differences in leaf functional traits (LFTs) in response to N addition under spatiotemporal or species classification variations. However, in N-rich forest ecosystems, it is unknown whether continuous N input regulates plant phenology, further strengthening or weakening these differences. Methods We examined how N addition at 0, 20 (LN), and 40 kg N hm–2 a–1 (HN) affected the variations in LFTs (and trait-trait covariations) among seasons (April and August) and plant types in an N-rich evergreen broadleaf forest in western China. Results Along the vertical vegetation gradient (from trees to shrubs to herbs), the seasonal variation in LFTs diminished. Most plants had higher C and P contents in August than in April. The HN treatment reduced the seasonal variation in C content of trees and herbs, while it accentuated that of shrubs. Additionally, HN significantly decreased the differences in C and P between trees and both shrubs and herbs, while enhancing the differences in leaf N between shrubs and herbs in August. However, N addition did not significantly affect leaf pH, dry matter content, or specific leaf area. Only the N–P scaling in covariations decreased with increasing N addition. Conclusion High N input may aggravate this area to move towards N saturation and P limitation. The heterogeneous response among plants with different ecological adaptation strategies to N additions regulating the differences in LFTs on seasonal dynamics as well as plant types, and could have important implications for plant–soil feedback mechanisms concerning nutrient limitation.

Publisher

Springer Science and Business Media LLC

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