Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging-Reference-Cited by-同舟云学术

Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging

Published:2024-02-14 Issue: Volume: Page:
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Author:

Ryu Sung Ho¹^ORCID,Kim Do-Hyeon²,Hong Minh Triet¹^ORCID,Lee Sun Hyeok²,Jazani Sina³,Jang Seongjae²,Seo Jongcheol²^ORCID,Ha Taekjip⁴^ORCID,Chang Young-Tae⁵^ORCID

Affiliation:

1. Pohang University of Science and Technology (POSTECH)

2. Pohang University of Science and Technology

3. Johns Hopkins University School of Medicine

4. Boston Children's Hospital and Harvard Medical School

5. Department of Chemistry, Pohang University of Science and Technology

Abstract

Abstract We introduce a super-photostable and bright organic dye, ‘Phoenix Fluor 555 (PF555)’, which exhibits an order-of-magnitude longer photobleaching lifetime than conventional organic dyes without the requirement of any anti-photobleaching additives. PF555 is an asymmetric cyanine structure where one side of indole from the conventional Cy3 is substituted with 3-oxo-quinoline. PF555 provides a powerful tool for long-term live-cell single-molecule imaging, as we demonstrated the dynamic interactions of the single-molecule epidermal growth factor receptor with clathrin-coated structures on the plasma membrane of a live cell under a physiological condition.

Publisher

Research Square Platform LLC

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