Adherence to and optimization of guidelines for Risk of Recurrence/Prosigna testing using a machine learning model: a Swedish multicenter study

Author:

Kjällquist Una1,Tsiknakis Nikolaos1,Acs Balazs1,Margolin Sara1,Kessler Luisa Edman2,Levy Scarlett2,Ekholm Maria3,Lundgren Christine3,Olsson Erik4,Lindman Henrik4,Valachis Antonios5,Hartman Johan1,Foukakis Theodoros1,Matikas Alexios1

Affiliation:

1. Karolinska Institutet

2. Saint Göran Hospital

3. Ryhov Hospital Jönköping

4. Uppsala University Hospital

5. Örebro University

Abstract

Abstract

Purpose Gene expression profiles are used for decision making in the adjuvant setting of hormone receptor positive, HER2 negative (HR+/HER2-) breast cancer. Previous studies have reported algorithms to optimize the use of RS/Oncotype Dx but no such efforts have focused on ROR/Prosigna. We sought to improve pe-selection of patients before testing using machine learning. Methods Postmenopausal women with resected HR+/HER2- node negative breast cancer tested with ROR/Prosigna in four Swedish regions were included (n = 348). We used the ROR/Prosigna assessment results to compare the performance of four risk classifications in terms of over- and undertreatment. We developed and validated a machine learning model that comprised simple prognostic factors (size, progesterone receptor expression, grade and Ki67) for prediction of ROR/Prosigna outcome. Results Adherence to guidelines reached 66.3%, with non-tested patients being older and having more comorbidities (p < 0.001). Previous risk classifications led to excessive undertreatments (CTS5: 21.8%, MINDACT/TailorX risk definitions: 28.1%) or large intermediate groups that would need to be tested with gene expression profiling (Ki67 cut-offs according to Plan B: 86.5%). The model achieved AUC under ROC for predicting ROR/Prosigna result of 0.77 in the training and 0.83 in the validation cohort. By setting and validating upper and lower cut-offs in the model, we could improve correct risk stratification and decrease the proportion of patients needing testing with ROR/Prosigna compared to current management. Conclusion We show the feasibility of machine learning algorithms to improve patient selection for gene expression profiling. Further validation in external cohorts is needed.

Publisher

Research Square Platform LLC

Reference29 articles.

1. Swedish Society of Pathology Quality and Standardization Committee (KVAST) Breast Cancer Guideline version 4. https://svfp.se/media/4jgbc3r2/kvastbilagabrost2022-02-17.pdf. Accessed on 26 January 2024.

2. Biomarkers for Adjuvant Endocrine and Chemotherapy in Early-Stage Breast Cancer: ASCO Guideline Update;Andre F;J Clin oncology: official J Am Soc Clin Oncol,2022

3. Screening older cancer patients: first evaluation of the G-8 geriatric screening tool;Bellera CA;Annals oncology: official J Eur Soc Med Oncol,2012

4. Molecular Drivers of Oncotype DX, Prosigna, EndoPredict, and the Breast Cancer Index: A TransATAC Study;Buus R;J Clin oncology: official J Am Soc Clin Oncol,2021

5. An updated PREDICT breast cancer prognostication and treatment benefit prediction model with independent validation;Candido Dos Reis FJ;Breast Cancer Res,2017

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