Genome-wide identification of trehalose-6-phosphate synthase (TPS) gene family reveals the potential role in carbohydrate metabolism in peach

Author:

Fan Shihao1,Wang Zhe1,Xiao Yuansong1,Liang Jiahui1,Zhao Shilong1,Liu Yihua2,Guo Jian1,Peng Futian1

Affiliation:

1. Shandong Agricultural University

2. Linyi University

Abstract

Abstract Background: Trehalose-6-phosphate synthase (TPS) is essential for plant growth and development, linking trehalose-6-phosphate (T6P) to carbon metabolism. However, little is known about the TPS gene family in peach and their potential roles in regulating carbohydrates in peach fruit. Results: In this study, 9 TPS genes were identified in peach genome and named according to the homologous genes in Arabidopsis. Phylogenetic analysis showed that three subfamilies were identified, including TPSI, TPSII-1 and TPSII-2, which were also consistent with gene structure analysis. Considerable cis-elements were enriched in the promoters, including plant hormone related elements. Tissue specific analysis showed that these TPS genes were mainly expressed in leaf, stem and fruit, showing different expression patterns for each gene. In addition, during fruit development, the content of T6P was positively correlated with the expression of PpTPS7a and negatively with SnRK1 enzyme activity. Transient overexpression and silencing of PpTPS7ain peach fruit validated its function in regulating T6P content and SnRK1 activity. Conclusions: The identified TPS genes and the potential function in regulating SnRK1 provide insights into carbohydrate metabolism in peach fruit.

Publisher

Research Square Platform LLC

Reference52 articles.

1. Trehalose in yeast, stress protectant rather than reserve carbohydrate;Wiemken A;Antonie Van Leeuwenhoek,1990

2. The function of trehalose biosynthesis in plants;Astrid W;Phytochemistry,2002

3. Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant;Miseon P;Int J Microbiol,2016

4. Mueller JBT, Wiemken A. Trehalose and trehalase in plants: recent developments. PLANT SCIENCE; 1995.

5. Olsson C, Jansson H, Swenson J. The Role of Trehalose for the Stabilization of Proteins. Journal of Physical Chemistry B 2016:acs.jpcb.6b02517.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3