CRYAB exerts anti-tumor effect as a favorable prognostic factor in prostate cancer

Abstract

Abstract Background: CRYAB is closely related to apoptosis, invasion and migration of tumor cells. Nevertheless, the relationship between CRYAB, outcomes of prostate cancer (PCa), and the potential mechanisms have not been studied. Material and Methods: The TPM format RNA sequencing (RNAseq) and methylation data on differential expression came from the cancer genome atlas (TCGA) database. R software and packages were used to analyze the different expression of CRYAB in TCGA database, the correlation between gene expression and immune cell infiltration, receiver operating characteristic curve, cox regression, Kaplan-Meier curve, nomogram models and calibration curve. Then, the bio-function of CRYAB in prostate cancer cell lines was detected via cell counting kit‑8, invasion and terminal deoxynucleotidyl transferase dUTP nick end labelling assay. Results: Compared to normal tissues, CRYAB was down-regulated in prostate cancer tissues based on the TCGA database, and was corrected with Gleason score. The promoter methylation level of CRYAB in PCa was significantly higher than that in normal. Immune cell infiltration analysis showed that the infiltration of all these immune cells except TReg was positively related to CRYAB expression. ROC curve and KM curve demonstrated that CRYAB had a meaningful diagnosis and prognostic value. Cox regression analysis indicated that higher T stage, N stage, PSA, Gleason score, and lower CRYAB expression were prognostic indicators of PCa. Cell function experiments suggested that up-regulated CRYAB can inhibit the proliferation, invasion and migration of prostate cancer cells, and promote apoptosis. Conclusion:We found that CRYAB was down-expressed in tumor tissues, and was related with anti-tumor function in PCa cells. We identified CRYAB had a significant diagnosis and prognostic value in PCa.