Effects of the TIBA and MH Antiauxins on In vitro Regeneration of Caspian honey locust

Abstract

Abstract In this study, the callus induction and shoot proliferation efficiency of Caspian honey locust (Gleditsia caspica Def.) root explants were investigated in response to 2,3,5-triiodobenzoic acid (TIBA) and maleic hydrazide acid (MH) antiauxins. TIBA and MH were applied in concentration of 0, 0.1, 1.0 and 10 μM along with 20 μM of BAP in MS medium. The maximum number of shoot proliferation (%), callus dry weight, callus fresh weight, callus growth rate, callus formation (%) and basal area was observed in 10 μM of TIBA and MH. The results showed that the most measured callus traits in root explants collected from proximal end of the root was much better than that of the distal end of the root explants. Regardless of explants position on root, callus formation percentage was also decreased in absence of TIBA and MH. In general, higher concentration of anti-auxin resulted in the better performance of callus traits and proliferation rate.