Affiliation:
1. Anhui Agricultural University
Abstract
Abstract
Background: Petunia hybrida is an important ornamental plant, and branch regulation is of great significance in its cultivation.
Results: We isolated the homologous gene of CYP724B1 from Petunia × hybrida cv 'Mitchell Diploid', and named PhCYP724B1. Expression analysis revealed that PhCYP724B1 was expressed highest in buds, while lowest in flowers. The expression level of PhCYP724B1 was higher in the petunia variety with more branches, while lower in variety with fewer branches. Subcellular localization assays demonstrated that PhCYP724B1 protein was localized on the endoplasmic reticulum. Furthermore, overexpression of PhCYP724B1 in transgenic Arabidopsis and petunia plants both enhanced shoot branching. In contrast, RNAi of PhCYP724B1 in petunia resulted in a significantly inhibited shoot branching phenotype.
Conclusions: Taken together, the results denote new insights into the important role of PhCYP724B1 in regulating petunia shoot branching.
Publisher
Research Square Platform LLC
Reference22 articles.
1. Isolation and characterization of a rice dwarf mutant with a defect in brassinosteroid biosynthesis[J];Mori M;Plant Physiol,2002
2. DWARF AND LOW-TILLERING, a new member of the GRAS family, plays positive roles in brassinosteroid signaling in rice[J];Tong H;Plant J,2009
3. Xia X, Dong H, Yin Y et al. Brassinosteroid signaling integrates multiple pathways to release apical dominance in tomato[J]. Proceedings of the National Academy of Sciences, 2021, 118(11): e2004384118.
4. Expression and functional analysis of ZmDWF4, an ortholog of Arabidopsis DWF4 from maize (Zea mays L.)[J];Liu T;Plant Cell Rep,2007
5. Strigolactones and brassinosteroids antagonistically regulate the stability of the D53–OsBZR1 complex to determine FC1 expression in rice tillering[J];Fang Z;Mol Plant,2020