Endurance of biologically active phytomarkers passing through classical drug preparation of Rauwolfia serpentina:An investigation by liquid chromatography in dual mode, planar and column. Names of authors

Author:

Hazra Kalyan1,Kumar Deepak1,Saha Subhodip1,Batule Manoj1,Mondal Susmita1,Debnath Sudipto1,Dahiya Jyoti1,Dutta Sreya1,Bolleddu Rajesh1,Mall Simmi1,Mandal Achintya2,Mangal Anupam Kumar1,Singh Ravindra3,Singh Arjun3,Babu Gajji1

Affiliation:

1. Central Ayurveda Research Institute

2. Siddha Central Research Institute

3. Central Council for Research in Ayurvedic Sciences

Abstract

Abstract Phytocompounds in medicinal plants attribute therapeutic values and are generally called biologically active markers. The fate of marker compounds comes under question when medicinal plants undergo different processes like crushing, grinding, boiling etc. These marker compounds may degrade or decompose and even attain some molecular rearrangement during these processes. As a result, medicinal plants' therapeutic activities may diminish or vanish altogether. Therefore, the efficacy of a particular dosage may not attain as it should possess, and that may be due to the change in the quantity of marker compounds present in the final product or formulation. The present study tried to determine the amount of specific marker reserpine in the root of the Rauwolfia serpentina and sarpagandha ghana satwa (an intermediate prepared from this plant) utilising densitometric HPTLC and HPLC-DAD. The qualitative presence of other secondary metabolites in root and ghana was screened through LC-MS/MS. The study revealed that the quantitative results of HPTLC (0.1742% & 0.1569% for root and ghana, respectively) and HPLC (0.1731% & 0.1535% for root and ghana, respectively) methods were almost similar, and there were no statistically significant differences between the mean values for the root and ghana. The LC-MS/MS data show that there are a few minor changes in the phytochemical array. The set of experiments revealed that secondary metabolites retain their properties in traditional processing.

Publisher

Research Square Platform LLC

Reference11 articles.

1. Drug development from natural products: Exploiting synergistic effects;Ulrich MG;Indian Journal of Experimental Biology,2010

2. Vaghbhatacharya (2010) Ashtanga Hridayam, Sutrasthana with Sarvangasundhara of Arunadutta and Ayurved Rasayana of Hemadri. Chukhamba surabharathi prakashan, Varanasi.

3. Sharangadhara AP (2018) Sharangadhara Samhita. Chaukhamba Surbharati Prakashan, Varanasi.

4. SA Kahalekar (2010) A practical approach to Ayurvedic Drug Manufacturing. Chowkhamba Bharti Academy, Varanasi.

5. Rauwolfia in the Treatment of Hypertension;Lobay D;Integrative Medicine,2015

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