Population variation and polymorphism of the Cotton Pink Bollworm Pectinophoragossypiella (Saunders) (Lepidoptera: Gelechiidae) using Inter Simple Sequence Repeats (ISSRs) as molecular marker

Abstract

Abstract The most common pest infesting Egyptian cotton plants is the pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae). Intensive insecticide applications required for effective control in production season, ensuing insecticide resistance problems. Some investigation ideas were floating for sponsored management. Thus, PCR based examination of pest population DNA diversity athrough differentiation using eight Inter-Simple Sequence Repeats (ISSRs) primers screening for genetic diversity and population differentiation between some Egyptian field populations Sharkia, Benisuef and Fayoum, based on similarity in the pattern of SDS-page bands. We detected 117 loci with an average population polymorphism of 97.64%. Genetic distance and similarity between groups were calculated and UPMGA phylogenetic tree were constructed and the primer efficiency were calculated. Principal component, correspondence analysis and AMOVA results revealed great variance between populations. Population features analysis provided moderate level of gene flow. Infinite diversity distinguished by Fixation index Fst across the genotypes in addition to number of identified alleles (Na) the effective number of alleles (Ne). Estimated Shannon’s index (I) procedures of calculations completed as inter-population differences and Nei’s genetic diversity (h) and Eveness plus the estimated diversity and species richness. All results indicates a high level of genetic variation exists among the three geographical sample populations of the same pest.