Xylanolytic Biotechnological Applications of Extracellular Xylanase Isolated from Marine Bacillus Subtilis AKM1

Abstract

Abstract Xylanase enzyme from marine bacteria was investigated for production improvement and studying its effect on agriculture wastes for use in biotechnological applications. By identification of the selective strain by using 16SRNA, the isolate was a new strain called Bacillus Subtilis AKM1 and has the accession number MK377251. The optimum culture conditions for xylanase production were as follows: incubation period (48 hrs.), initial pH (7.0), agitation speed (100 rpm), and incubation temperature(40°C). After purification, xylanase showed maximal activity at pH 7.0 and 50 °C. Metal ions such as Mn2+, Ca2+, and Fe2+ increased the residual activity by increasing the concentration, while Ag+, SDS, and EDTA completely inhibited the activity at higher concentrations. the molecular weight was 34 KDa, Km 0.044 mM, and Vmax 22.222. Xylooligosaccharides produced by enzymatic hydrolysis using the purified xylanase showed antimicrobial activity, antioxidant activity, and anti-tumor activity against breast and colon cancer cells.