Advances in the application of gene knockout technology in bacterial drug resistance research

Abstract

Abstract Gene knockdown has been confirmed as a common problem-solving method in biology. It is effective in investigating bacterial drug resistance. The technologies of gene knockdown comprise zinc-finger nuclease technology (ZFN technology), suicide plasmid vector systems, transcription activator-like effector protein nuclease technology (TALEN technology), Red homologous recombination technology, CRISPR/Cas, and so forth. To be specific, Red homologous recombination technology, CRISPR/Cas9 technology, and suicide plasmid vector systems have been the most extensively used technologies for the knockdown of bacterial drug resistance genes. The three above-described technologies have been employed in a considerable number of studies to obtain significant results in the research on bacterial gene functions. This study aims to provide an overview of effective gene knockout methods that are currently used for genetic drug resistance testing in bacteria and to provide a reference for the selection of gene knockout techniques.