Comparison of Chemical comstituents and content analysis in different parts of Eucommia ulmoides based on UPLC-Q-Orbitrap-MS and RP-HPLC

Abstract

Abstract Eucommia ulmoides is a valuable tonic Chinese herbal medicine with a long history. It has the effects of tonifying liver and kidney, strengthening muscles and bones, regulating Chong and Ren channels, consolidating meridians and preventing miscarriage. Modern pharmacological research has demonstrated that Eucommia ulmoides has the pharmacological effects of lowering blood lipids, lowering blood pressure, lowering blood sugar, preventing aging, and inhibiting oxidation. Currently, only the bark of Eucommia ulmoides can be utilized as a therapeutic component; the plant's overall consumption is still quite limited. The growth of Eucommia ulmoides bark is extremely sluggish, typically taking more than 12 years, and the greater technical needs of Eucommia ulmoides bark can easily result in the death of the tree, which is not favorable to the sustainable use of plant resources. In order to identify raw materials that can substitute Eucommia ulmoides bark, this experiment investigated the active components of different position of Eucommia ulmoides. Through ultraperformance liquid chromatography-quadrupole tandem mass spectrometry, the chemical constituents of several Eucommia ulmoides parts were examined in this work (UPLC-Q-Orbitrap-MS). The identification of 119 compounds of twelve known and novel kinds. In this investigation, a high performance liquid chromatography (HPLC) method for analyzing the quantity of active components in various Eucommia ulmoides parts was developed. The results showed that there were some differences in the components and contents of different parts of Eucommia ulmoides. The bark of Eucommia ulmoides included higher levels of geniposidic acid, pinoresinol diglucoside, and quercetin, whereas the leaves contained higher levels of chlorogenic acid, catechin, and rutin. The bark and Eucommia ulmoides had the highest concentrations of active components, followed by the remaining sections. Therefore, Eucommia ulmoides leaves can replace Eucommia ulmoides bark for anti-oxidation, anti-inflammatory, antibacterial, and glucose and lipid metabolism regulation. This research presents a novel method for evaluating the quality of Eucommia ulmoides.