Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi

Author:

Ao Keping1,Li Xiaohan1,Zhang Weili1,Chen Zhixing1,Liu Ya1,Shu Ling1,Xiao Yuling1,Wu Siying1,Xie Yi1,Kang Mei1

Affiliation:

1. West China Hospital of Sichuan University

Abstract

Abstract Background Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microbial identification; however, there is a paucity of data on its performance characteristics to identify filamentous fungi. The objective of our study was to evaluate the accuracy of the Autof ms1000 mass spectrometry for filamentous fungi identification in the clinical microbiology laboratory. Results Among 95.28% (101/106) that were identified using Autof ms1000 systems at Genus/Species, 76.41% (81/106) were correctly identified at the species level. In addition, a new rapid formic acid extraction method with simple pretreatment for filamentous fungi was developed to save time and obtain accurate results. Conclusions The Autof ms1000 mass spectrometer showed to be a useful instrument for filamentous fungi identification. Nevertheless, up-gradation of the database is recommended for the correct identification of rare strains.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3