Hepatoprotective effect of selenium nanoparticles coated with Spirulina polysaccharides against anti-tuberculosis drugs induced apoptosis

Abstract

Abstract This study aims to evaluate the ameliorating effect of selenium nanoparticles (SeNPs) coated with Spirulina polysaccharides (SPs-SeNPs) against anti-tuberculosis drugs (ATDs) induced hepatotoxicity and apoptosis in a trial to enhance the drug efficacy and tolerance, as well as to improve the chance of recovery of tuberculosis patients. The results revealed that utilizing SPs as a capping agent resulted in size-controlled, highly stable, and monodispersed spherical SeNPs with an average diameter < 50 nm. When compared to ATDs-treated animals, co-administration of either SPs (0.5mg /kg bw/day/8 weeks), SeNPs (0.5mg /kg bw/day/8 weeks) or SPs-SeNPs (0.5mg /kg bw/day/8 weeks) together with the ATDs[ ((Isoniazid (INH; 50 mg/kg) + Rifampicin (RIF;100 mg/kg) + Pyrazinamide (PZA;350 mg/kg)) /day/8 weeks ] for to rats modulated ATDs-induced side effects as evidenced by reductions in CYP2E1, CASPASE-3, malondialdehyde (MDA) concentration, liver function enzymes, levels of total bilirubin with elevation in total antioxidant capacity (TAC) and glutathione content (GSH) levels. These findings also demonstrated that treatment with SPs-SeNPs had a greater significant effect on ATDs-induced damage than treating with SPs or SeNPs alone. The results suggested that SPs–SeNPs could be a promising anti-toxic and antioxidant agent, and the strategy of using SPs as a surface decorator could be a very effective way to enhance the efficacy of nanomaterials.