Kaposi’s sarcoma-associated herpes virus (KSHV) may not be associated with substance abuse in oral cancer patients: A retrospective case review.

Abstract

Abstract Human Herpes virus 8 (HHV 8) better known as Kaposi’s sarcoma-associated herpes virus (KSHV) frequently relates to malignancies in acquired immune deficiency syndrome (AIDS) patients. Interestingly, one incident reported in a head and neck cancer patient who is also a HIV patient. In the light of these findings, this original research aimed to detect the HHV-8 status in oral squamous cell carcinoma tissues of Sri Lankan male patients. Incisional biopsies of cases were collected, transported, stored, and dispatched as frozen tissues at -800 C. A sub case sample selected from a large case control study to represent the vast majority of the oral cancer patients, in Sri Lankan context as oral cancer remains number 1 among Sri Lankan males. The DNA was extracted from frozen specimens using Gentra Puregene Tissue kit (Qiagen, Germany), solid tissue protocol according to the manufacturer’s instructions. The real-time PCR was performed with the specific primers to find out the possible association of this virus on substance abuse of OSCC patients in Sri Lanka. These data were obtained by a pre tested interviewer administered questioner were entered and analyzed using SPSS-21 Statistical Package. However, the HHV-8 DNA did not find in 22 OSCC cases. Kaposi’s sarcoma-associated herpes virus (KSHV) may not be associated with substance abuse in oral cancer patients. Powered longitudinal studies and invitro studies recommended to find out possible implication oral risk habits on this virus and activation of latent KSHV in B lymphocytes due to carcinogenic compounds in substances abused by OSCC patients.