Abstract
Background/Objectives: Ovarian cancer presents significant challenges in treatment efficacy, necessitating exploration of alternative therapeutic approaches. This study aimed to investigate the effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), particularly in conjunction with chemotherapy, on ovarian teratocarcinoma cells.
Subject/Methods: The study conducted rigorous cell viability assays to assess the impact of n-3 PUFAs on doxorubicin (DOXO)-induced cytotoxicity. Clonogenic assays, hanging drop assays, and apoptosis assays were employed to validate the observed effects. Network pharmacological analyses and molecular docking simulations were conducted to elucidate potential molecular mechanisms underlying the observed synergistic effects.
Results: Cell viability assays demonstrated a significant augmentation of DOXO-induced cytotoxicity by n-3 PUFAs, resulting in decreased cellular viability and migratory capacity. Clonogenic assays confirmed a reduction in colony formation in the combined treatment group, supported by additional experimental assays. Network pharmacological analyses identified topoisomerase II A (TOP2A) gene as a key target, while molecular docking simulations revealed structural analogies between n-3 PUFAs and DOXO, suggesting shared mechanisms of action.
Conclusion: The integration of computational and experimental approaches uncovered the synergistic effects of n-3 PUFAs and DOXO in ovarian cancer treatment. This study bridges the gap between theoretical understanding and practical application, offering promising prospects for enhanced therapeutic outcomes in ovarian cancer management.