Simultaneous quantification of 39 pesticide and veterinary drug residues in aquaculture products using ultra performance liquid chromatography tandem mass spectrometry with modified QuEChERS

Abstract

Abstract A high throughput quantification multi-residues method for the simultaneous detection of 39 pesticides and veterinary drugs in aquaculture products were developed by using Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) with modified QuEChERS (quick、easy、cheap、effective、rugged、safe). In order to balance recovery and detection efficiency, several parameters were optimized, including extracting, clarification, and purification. Chromatographic conditions were optimized to given run time of ＜ 13 min. Six representative matrices (muscle tissues of yellow croaker, pomfret, shrimp, white shell, flower shell, razor clam) show applicability with all target analytes showed recoveries (60.3%~119.0%) and acceptable precision (4.3%~20.8%). Furthermore, detection limits (LODs) and limits of quantification (LOQs) in the range of 1.0 ~ 5.0 µg/kg and 3.0 ~ 10.0 µg/kg, respectively, that providing a practical safety quantification method for internal risk controlling by aquaculture.