Affiliation:
1. The First School Clinical Medicine, Southern Medical University
2. Wuhan Fourth Hospital
Abstract
Abstract
Rotator cuff injury has always been a clinical challenge. Repairing rotator cuff injury with tissue engineering technique has become a commonly used research strategy. The purpose of this study is to construct a dynamic chondroitin sulfate and chitosan hydrogel scaffold (CHS) with bone morphogenetic protein 2 (BMP2), then seed tendon stem cells (TSCs) on BMP2-CHS for the rotator cuff reconstruction of tendon-to-bone interface. In this dynamic hydrogel system, the scaffold could not only have good biocompatibility and degradability but also significantly promote the proliferation and differentiation of TSCs. The ability of BMP2-CHS combined with TSCs to promote the regeneration of tendon-to-bone interface was further verified in the rabbit rotator cuff tear model. The results showed that BMP2-CHS combined with TSCs could induce considerable collagen, fibrocartilage, and bone arrangement and growth at the tendon-to-bone interface, and promote the biomechanical properties. Overall, TSCs seeded on CHS with BMP2 can enhance tendon-to-bone healing and provide a new possibility for improving the poor prognosis of rotator cuff surgery.
Publisher
Research Square Platform LLC
Reference43 articles.
1. Rehabilitation variability after rotator cuff repair;Galetta MD;J Shoulder Elbow Surg.,2021
2. Conditioned medium of human bone marrow-derived stem cells promotes tendon-bone healing of the rotator cuff in a rat model;Chen W;Biomaterials.,2021
3. Fenofibrate induces PPARalpha and BMP2 expression to stimulate osteoblast differentiation;Kim YH;Biochem Biophys Res Commun.,2019
4. BMP2 Is Required for Postnatal Maintenance of Osteochondral Tissues of the Temporomandibular Joint;O'Brien MH;Cartilage.,2021
5. Improved BMP2-CPC-stimulated osteogenesis in vitro and in vivo via modulation of macrophage polarization;Shen H;Mater Sci Eng C Mater Biol Appl.,2020