Identification of cuproptosis related genes in diagnosis and subtype classification of ALS using the Gene Expression Omnibus Database

Abstract

Abstract Cuproptosis is a newly discovered way of cell death which contributed to the accumulation of copper as well as targeting lipoylated TCA cycle proteins and what role that cuproptosis plays in ALS is still unknown. We analyzed 10 cuproptosis-related genes between ALS patients (233 samples) and non-ALS patients (508 samples) based on Gene Expression Omnibus (GEO) GSE112676. We constructed RF model to predict occurrence of ALS. By establishing coppercluster and gene cluster, we explored cuproptosis functioning patterns and immune cells infiltration in ALS and quantified these functioning patterns by erecting copperscore criterion. LIPT1, DLAT, DLD and PDHB were identified as differential expressed genes in ALS which high expression of them relates to pathogenicity of ALS. Moreover, T cell family, B cell family and dendritic family may highly be involved in the happening of ALS while mononuclear phagocyte system and nature killer cell family were silenced. Our findings provide potential immunotherapy and biomarkers to foresee the happening of ALS.