Novel silver nanocluster based on vancomycin antibiotic with peroxides like activity for colorimetric detection of S. aureus bacteria in milk samples

Abstract

Abstract A fast and colorimetric new strategy based on dual detection elements in one nanostructure as aptamer-silver nanocluster (apt-AgNC) is described for the specific and sensitive detection of Staphylococcus aureus (SA) based on silver nanocluster (AgNC) conjugated to oligonucleotide aptamer. AgNC is the first peroxidase activity research of NC based on vancomycin. This probe can specifically bind to the surface of SA and thus decrease the peroxidase activity of the apt-AgNC. Finally, in the presence of TMB as substrate and H2O2, the blue color of the solution in the infected milk samples was decreased. The detection limit for SA was 80 CFU mL− 1, and the processing time could occur during 45 min. This suggested SA detection technique has a number of appealing features, including high sensitivity, ease of use, quick testing time, and low cost.