Affiliation:
1. Center for Disease Control and Prevention of PLA
2. Anhui Medical University
Abstract
Abstract
Background
Human adenovirus (HAdV) infection causes acute respiratory diseases in immunocompetent individuals worldwide. Infections by HAdV-55, one of the most important pathogen leading to respiratory tract infections, often develop into pneumonia and are frequently associated with outbreaks in military camps and schools.
Methods
We studied 186 HAdV-55-positive nasopharyngeal swab samples that were collected from patients with respiratory infection in Wuhan, China, from 2018 and 2019. Fifteen samples with high viral loads were selected for viral isolation and cultured with MRC-5 cells. These strains were then whole-genome sequenced to investigate their genetic characteristics by phylogenetic analyses. We then selected three HAdV-55 strains to quantify viral replication and further assessed the pathogenic characteristics of one strain in Syrian hamsters and BALB/c nude mice.
Results
HAdV-55-174 exhibited the highest replication ability in cultured cells compared to other strains, so this strain was selected for further investigation. Although both the hamsters and mice were not so susceptible to HAdV-55-174 infection, viral loads were detected in the lungs and tracheas of the murine models, and persistent HAdV-55-174 replication was only observed in BALB/c nude mice. Histopathological lesions, including a widened alveolar septum and inflammatory cell infiltration, were observed in the lungs and tracheas of HAdV-55-174 infected animals.
Conclusion
In summary, we isolated and characterized the HAdV-55 strains circulating in Wuhan from 2018 to 2019, which may be meaningful for further studies on HAdV-55 infection and pathogenesis.
Publisher
Research Square Platform LLC
Reference40 articles.
1. Epidemiology, Global Spread of Novel Types, and Approach to Treatment;Lynch JP;Semin Respir Crit Care Med,2021
2. Outbreak of Febrile Respiratory Illness Associated with Adenovirus 11a Infection in a Singapore Military Training Camp;Kajon AE;J Clin Microbiol,2010
3. Outbreak of Febrile Respiratory Illness Caused by Adenovirus at a South Korean Military Training Facility: Clinical and Radiological Characteristics of Adenovirus Pneumonia;Hwang S-M;Jpn J Infect Dis,2013
4. Emergence of Community-Acquired Adenovirus Type 55 as a Cause of Community-Onset Pneumonia;Cao B;Chest,2014
5. Outbreak of Respiratory Illness Associated With Human Adenovirus Type 7 Among Persons Attending Officer Candidates School, Quantico, Virginia, 2017;Bautista-Gogel J;J Infect Dis,2019