Abstract
Background
CXCR6 + CD8 + T cells have been implicated in the pathogenesis of various liver and autoimmune diseases. However, their involvement in Primary Biliary Cholangitis (PBC) has not been eluciated.
Methods
We used immunohistochemistry and flow cytometry to quantify CXCR6 + CD8 + T cells in hepatic tissue and peripheral blood samples obtained from CXCR6 + CD8 + T cells obtained from PBC patients. Then, we performed comprehensive statistical analyses to access the correlation between the abundance of these cells and clinical as well as pathological data across different stages of PBC.
Results
Our research revealed that CXCR6 + cell frequencies in CD3 + CD8 + T cells from PBC patients significantly exceeded that of healthy controls (HCs) (2.24% vs. 0.61%, p < 0.01). A similar pattern emerged for hepatic CXCR6 + CD8 + T cell counts, which were notably higher in the PBC cohort compared to HCs. Our cohort consisted of 118 PBC patients, categorized into 62 early-stage (E-PBC) and 56 late-stage (L-PBC) cases. Notably, significant disparities existed between these groups in terms of liver enzyme and lipid profile levels (p < 0.05), with no notable differences observed in gender, age, blood counts, cholesterol levels, or autoantibodies (p > 0.05). Intriguingly, the quantity of hepatic CXCR6 + CD8 + T cells per high power field (HPF) was significantly elevated in both E-PBC and L-PBC patients as opposed to normal liver samples, indicating a substantial increase in these cells across all stages of PBC (p = 0.000). Spearman’s rank correlation analysis using showed a positive correlation between CXCR6 + CD8 + T cell counts and serum levels of Alkaline Phosphatase (AKP) and Gamma-Glutamyl Transferase (GGT), while revealing a negligible correlation with Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST). Subsequent findings indicated significant variances in CXCR6 + cell numbers not only among different PBC stages but also across various degrees of inflammation and fibrosis (p ≤ 0.007). In a follow-up study post-Ursodeoxycholic Acid (UDCA) treatment, stark differences were identified in biochemical and immunohistochemical profiles between responder (31 patients) and non-responder (33 patients) groups (p < 0.05). A Wilcoxon rank-sum test further demonstrated a significant difference in the level of hepatic CXCR6 + CD8 + T cells between these two response groups (p = 0.002).
Conclusion
CXCR6 + CD8 + T cells play a vital role in the pathogenesis of PBC, exhibiting correlations with the extent of inflammation, staging of liver fibrosis, and response to pharmacological interventions in PBC patients.