The Effect of Some Heavy Metals on the Growth of Garlic (Allium sativum L.) in In Vitro Conditions

Abstract

Abstract Background: Garlic is one of the important plant species due to both nutritional and medical importance. One of the important advantages of plant tissue culture is in vitro selection. Results: Therefore, in this study, it has been investigated the tolerance of garlic to some heavy metals under in vitro conditions. In vitro experiments were carried out in Murashige and Skoog (MS) nutrient medium including cadmium, cobalt, nickel, aluminum, copper, chromium and lead at 100 µm, 200 µm, 300 µm, 400 µm, 500 µm doses. During experimets, plant length, number of leaves, leaf length, root number and root length parameters were considered. Conclusions: When the results are evaluated, it has been observed decreases in both leaf and root development with the increasing doses of heavy metals. In addition, it has been found that the decrease in plant development at doses of 400 µm and 500 µm was higher than other doses and root formation did not observe in the high doses of cadmium, cobalt, nickel and copper (400 µm and 500 µm). It may be recommended to test the results of the study in vivoconditions.