Optimization of growth conditions of Streptomyces sp in Rock phosphate beneficiation process

Abstract

Abstract The current study is part of a project aimed at optimizing the growth conditions of the most bioactive Streptomces sp. strain isolated from rock phosphate waste (RW). Growth conditions include inoculum size, incubation time, temperature, initial pH, carbon source, and exposure period to UV ray on solid medium. This strain was found to grow efficiently when 1 ml of 1–2 X 10− 8 CFU inoculum excited with UV ray for up to 3 hours with inoculated in 1.0 kgl-1 RW for 7 days with an optimal temperature of 35˚C, pH 7 ± 0.2 on D-glucose. The processed RW is separated into 6 layers according to particle size. The P2O5% content increased from 16.28% to 18.80 P2O5% at room temperature and 22.30 P2O5% at 35˚C. Hence, this study focused on evaluating the role of Streptomyces in RW beneficiation. The results of Streptomyces growth on RW under ideal conditions showed beneficiation occurs in the rock composition according to the size of the particles, which leads to a redistribution of the P2O5% concentration in the layers. The importance of this research focusing on the role of Streptomyces RW biomining maximizes the benefits by controlling the conditions Streptomyces growth.