Viral replication probably promoted by EV71 VP4 protein via activating the PI3K/AKT and MAPK pathways in RD cell

Abstract

Abstract Enterovirus 71 (EV71) internal capsid protein (VP4) conserves in picornaviridae family and plays critical role in viral infection and replication by unknown mechanisms. In this study, mRNA sequencing was performed to analyze Differentially Expressional Genes (DEGs) in VP4 protein overexpressing Human Rhabdomyosarcoma Cells. It had been showed that Focal Adhesion, PI3K/AKT and MAPK pathways were mainly activated by the significant DEGs. Co-immunoprecipitation (Co-IP) assay indicated that ITGA5 of DEGs interacted with VP4. After knockdown of the ITGA5, WB showed that PI3K/AKT and MAPK pathways were activated, RT-qPCR experiments confirmed that viral copies were increasing. Thus, it is indicated that viral replication probably can be promoted by VP4 protein with activating the PI3K/AKT and MAPK pathways and a subsequent interaction with ITGA5.