Progonostic Effect of GSTM1/GSTT1 Polymorphism in Determining Cardiovascular Diseases Risk Among Type 2 Diabetes Patients in South Indian Population

Abstract

Abstract BACKGROUND Cardiovascular disease (CVD) is a significant complication of type 2 diabetes mellitus (T2DM), with oxidative stress playing a significant role. Glutathione S-transferase (GST) polymorphisms - GSTM1, GSTT1 - have been linked to CVD and T2DM. The role of GSTM1 and GSTT1 in CVD development among T2DM patients in the South Indian population is investigated in this study. MATERIALS AND METHODS The volunteers were grouped as Group 1: control, Group 2: T2DM, Group 3: CVD, and Group 4: T2DM with CVD (n = 100 each). Blood glucose, lipid profile, plasma GST, MDA, and total antioxidants were measured. GSTM1 and GSTT1 were genotyped using PCR. RESULTS GSTT1 plays a significant role in the development of T2DM and CVD [OR 2.96(1.64–5.33), < 0.001 and 3.05(1.67–5.58), < 0.001] while GSTM1 null genotype was not associated with disease development. Individuals with dual null GSTM1/GSTT1 genotype had the highest risk of developing CVD [3.70(1.50–9.11), 0.004]. Group 2 and 3 individuals showed higher lipid peroxidation and lower total antioxidant levels. Pathway analysis further indicated that GSTT1 significantly affects GST plasma levels. CONCLUSION GSTT1 null genotype may be considered as a factor for increasing the susceptibility and risk of CVD and T2DM in the South Indian population.