Abstract
Platelet adhesion and aggregation effect increases in third-trimester wemen, and the risk of thrombosis increases, so how to achieve early diagnosis is particularly important.In this study, microfluidic chip technology was used to study the adhesion and aggregation behavior of platelets in third-trimester under different arterial shear rates (1000s-1, 1500s-1, 4000s-1). Flow cytometry was used to analyze platelet surface activation markers (PAC-1 and P-selectin CD62P), and to explore the diagnostic value of different platelet function assessment methods for the risk of third-trimester thrombosis in normal pregnant women. Compared to healthy controls, white blood cell, fibrinogen, D-dimer levels increased, while platelet levels decreased (P < 0.05). No significant difference observed in platelet reactivity to agonist induction under static conditions ( P > 0.05). Platelet aggregation and surface activation marker expression significantly increased with the increase of shear rate under flow conditions (P < 0.05). The expression of platelet surface activation markers elevated.So we believe that using microfluidic chip technology to evaluate platelet aggregation and thrombosis in the third-trimester under arterial flow conditions combined with platelet activation can help predict thrombotic diseases. And the results may provide effective clinical application data and a theoretical basis for the diagnosis and prevention of platelet dysfunction and thrombotic diseases during pregnancy.