Antioxident and Anticancer Potency of Hydro-Alcoholic Ingudi (Balanites aegyptiaca Linn. Delile) Seed Extract

Abstract

Abstract Balanites aegyptiaca is a well-known medicinal plant, which has been traditionally used by local communities to treat various human ailments such as antibacterial, skin diseases, wound healing, and antioxidant properties. This study, examined the phytochemical composition and cell toxicity of Balanites aegyptiaca using established methodologies. The main objectives of this research work are: 1. Evaluation of hydro-alcoholic seed extract in cell viability in breast cancer cell lines. 2. The study significance antioxidant activity. 3. Phytochemical analysis of Balanites aegyptiaca extract. 4. Anticancer activity of Balanites aegyptiaca extract. The seeds were extracted using a hydro-alcoholic solvent (4:6 ratio), concentrated, and then lyophilized to obtain a dry crude extract. The radical scavenging capacity was determined using the DPPH method, while cytotoxicity was assessed through cell proliferation assays (MTT) involving MCF-7, MDA-MB-231, FaDu, and human PBMCs cells (2×103 cells). Our findings revealed that the hydro-alcoholic extract of the seeds exhibited significant antioxidant and anti-cancer properties. The novelty of this research work is the phytochemicals present in the plant extract that inhibit the migratory ability of breast cancer cells. The recommendations of this study can be adopted in the experimental and clinical trials.