Endurance effort affected expression the Actinin 3 and Klotho different isoforms in Arabian horses

Abstract

Background Among numerous genes that have been the focus of equine genetic research, the KL (Klotho) and ACTN3 (Alpha-actinin-3) genes stand out due to their significant roles in muscle function and overall health and performance ability. Previous studies on Arabian horses and other mammalians have shown that both KL and ACTN3 occur in different isoforms that seem to have different roles in metabolism. Methods and Results In present study the high throughput RNA analyses have been performed on a group of n = 10 Arabian horses taking a part in a long-distance 120km endurance ride. The Fold change (FC) values were calculated to describe the genes expression. Conclusions The results have shown surprisingly high differences between different isoforms expression level. Moreover, the correlation network analyses have shown the MIOX, SH3RH2 and TNNI2 genes as significantly involved in endurance effort metabolism.