Neural Reconnection After Exposure in High Frequency Electromagnetic Fields - In Vivo Study

Abstract

Change in cytoarchitecture and reconnection of neurons in rats’ brain under direct two months high frequency electromagnetic field (HFEMF) exposure has been found, supported with data and discussed. HFEMF used in the experiment had the following characteristics: 2.0 GHz frequency, 0.015 A/m intensity, electric field strength of 5.64 V/m, and SAR value was 2.5 W/kg. Rats were exposed for 6 hours a day for 6 days a week, during period of 60 days with experimental HFEMF. Six months after the treatment, histological and unbiased stereological analyses show that there is a reconnection of neurons in rats´ brain, demonstrated by slight increase in their number and production of new dendrites. Effects of HFEMF on neuronal disconnection were detected via caspase, while the SNXS25 protein indicated their reconnection and regeneration. Immunohistochemical analysis of nervous tissue in this paper shows that HFEMF disrupt the work of proteins found on cell membranes and endocytic function of neurons and thus interrupts interneuron communication. The advantage of HFEMF over other methods of treating brain damage is in their controlled and very precise use, with specifically defined characteristics of HFEMF, without ionizing and mutagenic effect on neurons.