Mycogenic Synthesis of gold nanoparticles mediated by the endophytic fungi Colletotrichum gloeosporioides, with special reference to In Vitro and In Silico studies

Abstract

Abstract The Mycogenic prospects of gold nanoparticles (AuNPs) using endophytic fungi C. gloeosporioides was evaluated. The AuNPs were confirmed and characterized by using UV –Vis spectroscopy with the shape and size of the nanoparticles ranging from 0.5 to 1 nm, depending on Scanning and Transmission microscopy. In addition, spot the capping factor responsible for the reduction of gold ions and the stabilization of nanoparticles prepared FTIR. The X-ray diffraction corresponds to the intense peaks at 2θ values recorded. The GCMS analysis studies showed the presence of active compounds Heptadecanoic acid, 16-methyl,-methyl ester AuNPs exhibited. In vitro anticancer activity against breast cancer cell line MCF-7 with different concentrations such as 100, 50, 25, 12.5, 6.25, 3, 3.125, 1.562 and 0.781 μg/ml were treated. The cell viability in the percentage of the respective concentration was about 15.33 to 77.90. The synthesize synthesized mycogenic associated nanoparticles derived active major compound Heptadecanoic acid, 16-methyl,-methyl ester ligand was indicate good activity against BRCA 1 and HER 2 breast cancer cell line protein receptor. The present investigation reports it gives a clear role in the therapeutic and biocatalytic contribution of synthesized mycogenic gold nanoparticles.