Antibiotic susceptibility and resistance of P. aeruginosa uropathogenic isolates

Abstract

Abstract Urinary tract infections (UTIs) are one of the most common infectious morbidities among hospitalized patients. Pseudomonas aeruginosa is an important opportunistic human pathogen responsible for the many human infectious diseases. Although, P. aeruginosa provokes only 7–10% of healthcare-associated UTIs, its flexible biochemical and adaptive mechanisms results in the high antibiotic resistance, followed by the increased mortality of patients that makes P. aeruginosaextremely dangerous. A resistance of P. aeruginosa to different classes of antibiotics related to a broad range of its intrinsic, acquired, and adaptive mechanisms including expression of specific β-lactamases. Herein, we studied antibiotic susceptibility of 22 P. aeruginosa urine isolates and the occurrence of metallo-β-lactamases genes in their genomes as one of the main factors of a high resistance to carbapenems. It was shown that all studied P. aeruginosa isolates were resistant or susceptible in increased exposure to carbapenems (imipenem, ceftazidime, and piperacillin/tazobactam) and fluoroquinolone ciprofloxacin. The only effective carbapenem were meropenem, showed inhibition activity against seven isolates. A sensitivity to aminoglycoside class antibiotic amikacin was detected in 82% of the isolates. Only six of isolates contained VIM-type metallo-β-lactamase genes in their genomes.