Cryo-EM structure of native honey bee vitellogenin

Author:

Luecke Hartmut1ORCID,Montserrat-Canals Mateu2,Schnelle Kilian3ORCID,Moeller Arne4ORCID,Amdam Gro5,Halskau Øyvind6,Leipart Vilde7,Cunha Eva S.8

Affiliation:

1. Nova University Lisbon

2. University of Oslo

3. University of Osnabrück

4. Osnabrueck University

5. Arizona State University

6. University of Bergen

7. Norwegian University of Life Sciences

8. Proteros Biostructures GmbH

Abstract

Abstract

Vitellogenin (Vg) is the main yolk precursor lipoprotein in almost all egg-laying animals. In addition, along its evolutionary history, Vg has developed a range of new functions in different taxa. In the honey bee, Vg has functions related to immunity, antioxidant protection, social behavior and longevity. However, the molecular mechanisms underlying Vg functionalities are still poorly understood. Here, we report the cryo-EM structure of full-length honey bee Vg, onestep purified directly from hemolymph. The structure provides structural insights into the overall domain architecture, including the lipid binding cavity and the previously uncharacterized von Willebrand factor type D domain. A domain of unknown function has been identified as a C-terminal cystine knot domain based on structural homology. Information about post-translational modifications, cleavage products, metal and lipid binding allow an improved understanding of the mechanisms underlying the range of Vg functionalities. The findings have numerous implications for the structure-function relationship of vitellogenins of other species as well as members of the same protein superfamily, which share the same structural elements.

Publisher

Springer Science and Business Media LLC

Reference78 articles.

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