Abstract
Purpose-
Cypermethrin (CYP) is a commonly used type II Pyrethroid. This pesticide tends to accumulate in the various body tissues causing organ dysfunction. The current study is primarily focused on investigating cypermethrin-induced hypothyroidism and the potential mitigating effect of Bacopa monnieri extract in female mice.
Method-
Animals were divided into four groups, each comprising 6 mice. Group I served as a control, Group II was orally exposed to CYP at 15 mg/kg, while Group III animals were administered with Bacopa monnieri (BM) extract at a dose of 200 mg/Kg and Group IV received CYP as well as Bacopa monnieri extract for 28 days by oral route. At the end, serum levels of triiodothyronine (T3), thyroxin(T4), and thyroid stimulating hormone (TSH) were measured. Results- The thyroid histopathological examination and tissue peroxidation were studied which revealed the CYP-induced deformed follicles with flattened epithelium and reduced diameter, vacuolated cytoplasm, and decreased expression of THR beta-1. In this group, a marked elevation in TSH and a considerable decline in both T3 and T4 levels were observed. Moreover, a significant increase in the bio-morphometric indices (body weight and thyroid weight) was seen. Marked morphometric abnormalities and hormonal dysregulation indicated the CPY-induced hypothyroid condition. Interestingly, the co-administration of Bacopa monnieri extract and CYP showed a positive result as it could ameliorate hypothyroidism in mice. Results of biochemical assessments revealed increased oxidative stress in the thyroid gland by CYP, reversed in the CYP + BM group.
Conclusion-
These results suggest the mitigative effect of the test drug on cypermethrin-induced hypothyroidism. This work demonstrates an entirely novel expression of THR beta-1 and oxidative stress in the context of hypothyroidism that has been generated.