Aerobic training with consumption of Nigella sativa extract ameliorate myelin core protein, axonal regeneration, and cognitive function in a mouse model of multiple sclerosis

Abstract

Abstract Purpose Multiple sclerosis (MS) is a progressive disease of the central nervous system (CNS) that leads to demyelination of the CNS and memory impairment in animals suffering from this disease. It seems that the release of neurotrophic factors after training is one of the possible mechanisms for increasing axonal protection and brain health of these patients. methods In order to induce MS, cuprizone was added to rodent food powder at a weight ratio of 0.2%. The state of balance in the studied groups was checked by repeated measurement statistical test, the level of expression of brain derived neurotrophic factor (BDNF) and myelin basic protein (MBP) markers by immunohistochemistry, the amount of tumor necrosis factor-alpha (TNF-α) by ELISA method and the evaluation of memory and spatial learning by Morris water maze test. Results The amount of MBP in the corpus callosum in the cuprizone (CUP) group was significantly decreased compared to the sham group and significantly increased in the groups treated with training and oral Nigella sativa compared to the CUP group. A significant increase in BDNF and a significant decrease in TNF-α were observed in the treated groups compared to the CUP group. Conclusion probably due to the reduction of inflammation and the improvement of myelin, the balance and motor activity of the animals have also improved. These structural changes in the brain anatomy of mice, which show histological changes and in line with its behavioral function, can have a new path for faster diagnosis and prevention of debilitating symptoms.