Detoxification effects and potential mechanisms of NP-induced hepatotoxicity by ethanolic extracts from Clinacanthus nutans (Burm.f.) Lindau leaf

Author:

Xiao Xueman1,Chen Congying1,Huang Huiying1,Shi Jian1,Liu Huan1,Liu Chunhong1

Affiliation:

1. South China Agricultural University

Abstract

Abstract Clinacanthus nutans (Burm.f.) Lindau (C. nutans) is native to tropical Asian countries and is an important ingredient in the traditional medicinal system. It is of interest because of the flavonoids it contains, which are rare in plants. It has traditionally been used to treat herpes infections and snake venoms. However, the ability of C. nutans to counteract exogenous chemical-induced hepatotoxicity is not yet known. Nonylphenol (NP) is a widely distributed environmental endocrine disruptor, and the liver is one of the target organs for its toxic effects. In this study, we designed in vitro tests to investigate the protective effect of ethanolic extracts from C. nutans leaf (CE) on NP-induced hepatotoxicity and its molecular mechanism. Sixty µM NP was selected as the optimum to treat Hep G2 cells for 24 h to establish a cell injury model, and the intervention doses of CE were 0.25, 0.5, 1, 2 mg/mL, followed by ERK inhibitor (PD98059), P38 inhibitor (SB202190), Nrf2 inhibitor (ML385) to assist in validation of the intervention mechanism. The results showed that CE could reverse NP-induced Hep G2 cytotoxicity and upregulated cell survival, cellular GSH content, CAT viability and ATP content which was probably achieved by inhibiting ERK/P38 mitogen-activated protein kinases (MAPKs) pathway and activating the nuclear erythroid 2-related factor 2/Heme Oxygenase-1 (Nrf2/HO-1) pathway. These data suggest that CE play a detoxifying role in NP-induced hepatotoxicity, which may be achieved by inhibiting the ERK/P38 MAPKs pathway and activating the Nrf2/HO-1 pathway.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3