Reidentification of the causal agent and addition of new symptoms of black leaf blight in fig tree in Japan

Author:

Kikuhara Kenji1ORCID,Inoue Kouji2,Motohashi Kenichi3

Affiliation:

1. Agriculture Forestry and Fisheries Research Information Technology Center: Norin Suisan Kenkyu Joho Sogo Center

2. Okayama-ken

3. Tokyo University of Agriculture: Tokyo Nogyo Daigaku

Abstract

Abstract In August 2007, twig blight, branch canker, dieback, and fruit rot were observed in fig trees (cv. Toyomitsuhime) with black leaf blight in Japan. All isolates from each symptomatic part were identified as the fungi causing this disease. These symptoms were reproduced after inoculating their isolates; isolates were then reisolated. We demonstrated this fungus caused new symptoms. The isolates were re-evaluated based on morphological characteristics and phylogenetic analysis of concatenated rDNA-ITS, EF1-α, TUB2, and RPB2 partial sequences. They were phylogenetically classified as a new independent clade. We propose a new species of the fungus as Neofusicoccum ficicola.

Publisher

Research Square Platform LLC

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5. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes;Glass NL;Appl Environ Microbiol,1995

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