Affiliation:
1. Tehran University of Medical Sciences
2. Tarbiat Modares University Faculty of Medical Sciences
3. Tarbiat Modares University Faculty of Biological Sciences
4. Semnan University of Medical Sciences and Health Services
Abstract
Abstract
Noise and combination-induced hearing loss is a common work-related disease among workers. The present research aimed to investigate the impact of independent and combined acute exposure to white noise plus Al2O3-NPs on changes in hearing and structural functions of the cochlea in rats. A total of 36 rats were randomly classified into six groups: Control, acute exposure to white noise, exposure to Al2O3-NPs (γ), exposure to noise plus Al2O3-NPs (γ ), exposure to Al2O3-NPs (α), and exposure to the combination of noise plus Al2O3-NPs (α). TTS and PTS were examined using DPOAE. Oxidative index (MDA, GSH-Px), gene expression (NOX3, TGF-ß, CYP1A1), protein expression (ß-Tubulin, Myosin VII), and histopathological changes were examined in the cochlea. The morphology of AL2O3-NPs was examined by TEM. The results of the DPOAE test, TTS in all groups, and the PTS in the groups exposed to noise, Al2O3-NPs (γ), and a combination of noise plus Al2O3-NPs increased significantly (p < 0.05). In exposure to white noise plus Al2O3-NPs, the MDA levels increased, the level of GSH-Px decreased, the expression percentage of ß-Tubulin and Myosin VII decreased, and the expression of NOX3, TGF-ß, and CYP1A1, (except for AL2O3-NPs (α) group) significantly increased (p < 0.05). Histopathological changes of the cochlea indicated damage in hair and ganglion cells which was higher in the combined exposures. The combined and independent exposure to white noise plus Al2O3-NPs damaged hair and ganglion cells for high-frequency perception, thereby affecting the function and structure of the cochlea and leading to TTS and PTS.
Publisher
Research Square Platform LLC
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