Functional assessment of Zinc oxide nanoparticle and Moringa oleifera supplementation on the male reproductive system of a diabetic rat model

Abstract

Abstract The probable synergistic effect of chemically synthesized zinc oxide nanoparticles and locally secured Moringa oliefera was assessed on diabetic rats’ model along with a potential effect on the male reproductive system and rat male serological markers. The plant Moringa oliefera was procured from the University of Agriculture Faisalabad (UAF) step solvothermal method was utilized to synthesize the zinc oxide nanoparticles. The zeta sizer, UV-vis, and spectroscopic analysis of ZnO nanoparticles were undertaken. A total of fifty healthy male albino Wistar rats weighing 250 ± 10 g were used in the experimental research study having been divided into six stops designated as negative control (NC), positive control (PC), standard control (Std. C), ZnO na nanoparticle group (ZnO), Moringa oliefera. Group (MO) and a group of combinations thereof (ZnONP + MO). The mean body weight was observed to be significantly normalized in the group ZnONP + MO i.e., 230 ± 6 grams in contrast to PC i.e., 162grams ± 4; P ≤ 0.05. The ZnONP + MO combination had a normoglycemic effect i.e., 154.4 ± 4.5 mg/dl as in opposition to the positive control, i.e., 315.7 ± 3 mg/dl. The serum level of rat testosterone in the ZnONP + MO group was observed at 0 .958 ± .08ng/ml in opposition to post-itive control, which revealed it at 0.442 ± .02 ng/ml). The follicle-stimulating hormone level in the ZnONP + MO group was recorded at 10.04 ± .04 mlU/ml significantly varied from the PC, whose level was noted at 5.08 ± .09 mlU/ml. The level of LH in the ZnONP + MO group was observed at 6.89 ± .08 mIU/ml, significantly different from PC at 3.78 ± .08 mIU/ml. Histopathological changes in the rat testes treated with alloxan alone revealed the distortion in the epithelium of seminiferous tubules (H&E stain). However, the histopathology of testes isolated from rats treated with the zinc oxide nanoparticle and Moringa oliefera combination showed almost normal spermatogenic activity, the lumen of seminiferous tubules contained sperms, normal spermatids, outer epithelium layer of seminiferous tubules was intact.