Affiliation:
1. Tokyo Women’s Medical University, Adachi Medical Center
2. Juntendo University Graduate School of Medicine
3. Duke University School of Medicine
Abstract
Abstract
This in vitro study evaluated the potential hemostatic effect of fresh frozen plasma (FFP) ultrafiltration on clotting factors, coagulation parameters, and plasma properties. ABO-specific units of FFP (n = 40) were prepared for the concentrated FFP and cryoprecipitate. Plasma water was removed from FFP by ultrafiltration using a dialyzer with a pump running at a 300 ml/min. The aliquot of each concentrated FFP after 50, 100, 200, and 250 ml of fluid removal were served for the standard coagulation assay, measurement of clotting activity, and plasma properties to compare those parameters of cryoprecipitate. Concentrated FFP contained 36.5% of fibrinogen in FFP with a mean concentration of 7.2 g/L, lower than the cryoprecipitate level. The levels of factor Ⅷ (FⅧ), von Willebrand factor (vWF): antigen (Ag), and vWF: ristocetin cofactor (Rco) were also lower in concentrated FFP, whereas the levels of factor Ⅴ, factor Ⅸ, factor ⅩⅢ, antithrombin and albumin was higher in concentrated FFP. Maximum clot firmness (MCF) in thromboelastometry was approximately one-half of that in cryoprecipitate. Although the levels of vWF: Ag, vWF: Rco, and FⅧ differed depending on the ABO blood types, fibrinogen levels, and MCF were not significantly different among the ABO blood groups in FFP and concentrated FFP.
Publisher
Research Square Platform LLC
Reference41 articles.
1. Levy JH, Welsby I, Goodnough LT. Fibrinogen as a therapeutic target for bleeding: a review of critical levels and replacement therapy. Transfusion. 54, 1389 – 405(2014)
2. Mechanisms of thrombus formation;Furie B;N Engl J Med,2008
3. The European guideline on management of major bleeding and coagulopathy following trauma: fifth edition;Spahn DR;Crit Care,2019
4. Nascimento B, Goodnough LT, Levy JH. Cryoprecipitate therapy. Br J Anaesth 113, 922 – 34 (2014)
5. protocol for the in-house production of cryoprecipitate;Ohnish K;Jpn J Transf Cell Ther,2016