Taxonogenomics of Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov.: two new bacterial species isolated from human stool microbiota

Abstract

Abstract Two new bacterial strains, Marseille-P2698 (= CSUR P2698 = DSM 103121) and Marseille-P2260 (CSUR P2260 = DSM 101844 = SN18), were isolated from human stools by the culturomic method. We used the taxonogenomic approach, to fully describe these two new bacterial strains. The Marseille-P2698 strain was a Gram-negative, motile, non-spore-forming, rod-shaped bacterium. The Marseille-P2260 strain was a Gram-positive, motile, spore-forming rod-shaped bacterium. Major fatty acids found in Marseille-P2698 were C15:0 iso (63%), C15:0 anteiso (11%), and C17:0 3-OH iso (8%). Those found in Marseille-P2260 strain were C16:00 (39%), C18:1n9 (16%) and C18:1n7 (14%). Strains Marseille-P2698 and Marseille-P2260 had 16S rRNA gene sequence similarities of 91.50% with Odoribacter laneus, and of 90.98% and 95.07% with Odoribacter splanchnicus and Eubacterium sulci, respectively. The exhibited digital DNA-DNA Hybridization values lower than 20.7%, and Orthologous Average Nucleotide Identity values lower than 73% compared to their closest related bacterial species O. splanchnicus and E. sulci respectively. Phenotypic, biochemical, phylogenetic, and genomic results obtained by comparative analyses provided sufficient evidence that both of the two studied strains Marseille-P2698 and Marseille-P2260 are new bacterial species for which the names Culturomica massiliensis gen. nov., sp. nov., and Emergencia timonensis gen. nov., sp. nov. were proposed, respectively.