Voltammetric Determination of Acrylamide Using Coal Tar Pitch Modified Pencil Graphite Electrode by SWV

Abstract

Abstract The adverse effects of acrylamide (AA) on humans are being clear, especially after a series of related investigations reported the dependance of consuming foods that prepared by exposing to high temperature for a long-time and cancer risk. Accurate determination of AA in food samples at trace amount is considered the first step to overcome this significant problem. The determination of AA using coal tar pitch modified pencil graphite (PGE/CTP) electrode was reported. The electrochemical behavior of AA was studied on PGE/CTP electrode in different medium acidities (pH) of phosphate and Briton-Robinson (BR) buffer solutions by employing square wave voltammetry (SWV). Linear sweep voltammetry (LSV) technique was applied to determine the mass transfer mode of AA from bulk solution to the PGE/CTP electrode surface. The optimum conditions were using phosphate buffer solution (PBS) at pH 7.0. The linear relationship among peak current and AA concentration was in the range 1000.0 nM to 0.5 nM. The limit of detection for detection of AA was 0.2094 nM and the limit of quantitation for quantification was 0.6912 nM. In addition, the PGE/CTP electrode as the sensor was successfully used for the determination of AA in instant coffee sample.