Chandipura virus nucleoprotein interact with heat shock cognate 71 protein in Neuro-2a cells during replication

Author:

Kavathekar Vishal K1,Sambhare Susmit2,Anukumar Balakrishanan1

Affiliation:

1. National Institute of Virology, Govt. T. D. Medical College Hospital

2. National Institute of Virology

Abstract

Abstract To complete their lifecycle, viruses interacts with variety of cellular proteins. Identifying host proteins involved in the viral lifecycle is an excellent target for drug. In neuro-2a cells, we employed recombinant Chandipura virus (CHPV) nucleoprotein (N) as bait in a protein pull down assay to see which cellular proteins interact with nucleoprotein. A total of ten proteins interact with the CHPV N protein. Out of ten proteins, heat shock cognate 71 (HSC70) protein was investigated further. In CHPV-infected neuro-2a cells, confocal microscopy revealed that HSC70 co-localized with CHPV N protein, and that the expression was altered by viral infection. The association with HSC70 may help to mitigate the negative repercussions of misfolded proteins produced by viral polymerase's erroneous nature. More research on the role of these proteins in viral replication in infected cells is needed.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3