Development of serum lipoprotein (a) detection using latex enhanced immunoturbidimetry

Abstract

Background Lipoprotein (a) (Lp (a)) is indeed a significant factor in cardiovascular health, as it is a product of low-density lipoprotein cholesterol-like particles that bind to apolipoprotein (a). Elevated levels of Lp (a) have been linked to an increased risk of cardiovascular diseases (CVD), hastening disease progression and raising CVD mortality rates. However, the absence of standardized measurement methods for Lp (a) contributes to diagnostic uncertainties. Method A quantitative measurement method for serum Lp (a) was developed using fully automated latex-enhanced particle immunoturbidimetry technology represents a significant advancement in diagnostic capabilities. The key parameters such as repeatability, stability, linearity, and method comparison were evaluated to ensure the accuracy of the assay. Result The Lp (a) in samples was recognized by carboxylated latex particles covalently coated with anti-Lp (a) antibodies. The content of Lp (a) was quantified by measuring the changes in turbidity generated by agglutination at 600 nm. With precision CV% within the batch of 1.10% and inter-batch precision CV% of 1.79%, it demonstrates reliable performance using Randox biochemical quality control samples. The detection limit of 7 mg/L and a high correlation coefficient (R² = 0.9946) at concentrations of 0-1500 mg/L further validate its effectiveness. Conclusion The quantitative determination method of serum Lp (a) based on latex-enhanced immunoturbidimetric analysis indeed provides rapid results, high accuracy, and automation, making it suitable for routine clinical testing. This method relies on the interaction between Lp (a) and latex particles, allowing for efficient measurement in serum samples.